Raman spectroscopy and diffuse reflectance spectroscopy for diagnosis of human cancer and acanthosis nigricans
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چکیده
RAMAN SPECTROSCOPY AND DIFFUSE REFLECTANCE SPECTROSCOPY FORDIAGNOSIS OF HUMAN CANCER AND ACANTHOSIS NIGRICANSby SUNEETHA DEVPURAAugust 2012Advisor : Dr. Ratna NaikCo-Advisor : Dr. Steven J. RehseMajor : Physics (Condensed Matter)Degree : Doctor of Philosophy Cancer and diabetes are common chronic diseases in today’s world causingnumerous deaths in adults as well as children. Most common types of cancers in adultsinclude prostate, lung, breast, colorectal and head and neck squamous cell carcinoma, whileamong children; leukemia, and brain and central nervous system cancers are quitecommon. In each of these cases, early detection of the cancer or disease dramaticallyincreases the chances of successful treatment. In recent years, there has been muchinterest in using Raman spectroscopy and diffuse reflectance spectroscopy as analytical optical spectroscopic methods for early diagnosis of diseases. Raman spectroscopy can beused to measure changes in the bio-molecular composition of a tissue specimen, and diffusereflectance spectroscopy can measure chromophores of the skin. In this research, archived(formalin-fixed paraffin processed) tissues of head and neck squamous cell carcinoma,prostate, and pediatric tumors have been investigated using Raman spectroscopy. We haveutilized statistical methods such as principal component analysis (PCA) and discriminantfunction analysis (DFA) to analyze the spectral output and distinguish between normal and
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